Dative intervention is a gang effect

This paper addresses two restrictions regarding agreement with nominative arguments in Icelandic DAT-NOM constructions. The first is the reported asymmetry in intervention effects in mono-clausal versus bi-clausal environments. The second regards the well-known Person Restriction that prohibits agreement with non-3rd person arguments. It is argued that both of these phenomena can be viewed as instances of cumulative constraint interaction, where less important constraints in the grammar ‘gang up’ to block some higher constraint. In order to account for this, I adopt a model of syntax with both weighted constraints and serial optimization that is known as Serial Harmonic Grammar in the phonological literature. It will be demonstrated that such a system can offer a more principled analysis of the construction-specific nature of the aforementioned phenome

Resveratrol Prevents High Fluence Red Light-Emitting Diode Reactive Oxygen Species-Mediated Photoinhibition of Human Skin Fibroblast Migration.

BackgroundSkin fibrosis is a significant medical problem that leads to a functional, aesthetic, and psychosocial impact on quality-of-life. Light-emitting diode-generated 633-nm red light (LED-RL) is part of the visible light spectrum that is not known to cause DNA damage and is considered a safe, non-invasive, inexpensive, and portable potential alternative to ultraviolet phototherapy that may change the treatment paradigm of fibrotic skin disease.ObjectiveThe goal of our study was to investigate the how reactive oxygen species (ROS) free radicals generated by high fluence LED-RL inhibit the migration of skin fibroblasts, the main cell type involved in skin fibrosis. Fibroblast migration speed is increased in skin fibrosis, and we studied cellular migration speed of cultured human skin fibroblasts as a surrogate measure of high fluence LED-RL effect on fibroblast function. To ascertain the inhibitory role of LED-RL generated ROS on migration speed, we hypothesized that resveratrol, a potent antioxidant, could prevent the photoinhibitory effects of high fluence LED-RL on fibroblast migration speed.MethodsHigh fluence LED-RL generated ROS were measured by flow cytometry analysis using dihydrorhodamine (DHR). For purposes of comparison, we assessed the effects of ROS generated by hydrogen peroxide (H2O2) on fibroblast migration speed and the ability of resveratrol, a well known antioxidant, to prevent LED-RL and H2O2 generated ROS-associated changes in fibroblast migration speed. To determine whether resveratrol could prevent the high fluence LED-RL ROS-mediated photoinhibition of human skin fibroblast migration, treated cells were incubated with resveratrol at concentrations of 0.0001% and 0.001% for 24 hours, irradiated with high fluences LED-RL of 480, 640, and 800 J/cm2.ResultsHigh fluence LED-RL increases intracellular fibroblast ROS and decreases fibroblast migration speed. LED-RL at 480, 640 and 800 J/cm2 increased ROS levels to 132.8%, 151.0%, and 158.4% relative to matched controls, respectively. These LED-RL associated increases in ROS were prevented by pretreating cells with 0.0001% or 0.001% resveratrol. Next, we quantified the effect of hydrogen peroxide (H2O2)-associated ROS on fibroblast migration speed, and found that while H2O2-associated ROS significantly decreased relative fibroblast migration speed, pretreatment with 0.0001% or 0.001% resveratrol significantly prevented the decreases in migration speed. Furthermore, we found that LED-RL at 480, 640 and 800 J/cm2 decreased fibroblast migration speed to 83.0%, 74.4%, and 68.6% relative to matched controls, respectively. We hypothesized that these decreases in fibroblast migration speed were due to associated increases in ROS generation. Pretreatment with 0.0001% and 0.001% resveratrol prevented the LED-RL associated decreases in migration speed.ConclusionHigh fluence LED-RL increases ROS and is associated with decreased fibroblast migration speed. We provide mechanistic support that the decreased migration speed associated with high fluence LED-RL is mediated by ROS, by demonstrating that resveratrol prevents high fluence LED-RL associated migration speed change. These data lend support to an increasing scientific body of evidence that high fluence LED-RL has anti-fibrotic properties. We hypothesize that our findings may result in a greater understanding of the fundamental mechanisms underlying visible light interaction with skin and we anticipate clinicians and other researchers may utilize these pathways for patient benefit

Computational Analysis of Flow Around a Scaled Axial-Flow Hydro Turbine

Water power energy harvesting has become an important and growing industry supported by research and significant interest and impetus from the government and commercial sector. Due to waters high power density, marine current energy is an attractive addition to other renewable energy technologies. That said, there is still much to be understood about the design, application, installation, operation, and maintenance of current-driven marine-hydrokinetic devices and with new designs coming out frequently it is imperative to understand the physics and dynamics involved. Recently, testing of the Sandia turbine, designed by University of California, Davis, Pennsylvania State and Sandia National Laboratories, took place at Pennsylvania State\u27s Applied Research Laboratory. The testing was performed with a 1:8.7 scale model of the Sandia turbine. The experiment successfully assessed the turbine\u27s power and cavitation performance, unsteady driveshaft loading, blade strain, unsteady tower pressure, flow field, and acoustics. Given the expense and time required for experimentation, commercial computational fluid dynamics codes, such as CD-Adapco\u27s Star-CCM+ can be used to simulate new designs both quickly and inexpensively. The goal of this research is to validate the results obtained using Star CCM+ to model the Sandia turbine as compared to the experimental results obtained by Pennsylvania State. To carry out the simulation, several unstructured grids were generated on which several unsteady simulations were run at the design point of a tip-speed ratio of 4. For this design point at 95% blade span, the chord Reynolds number is approximately 500,000 which equates to about 2,000,000 for the full scale turbine. In comparing model results to experimental data there was good agreement for power performance parameters, such as thrust and power coefficients, as well as downstream velocity profiles. With such good agreement it is optimistic that computational fluid dynamics models can be used to accurately predict the performance of future water turbine designs, thus reducing the high cost of research needed to develop novel current-driven marine-hydrokinetic devices.\u2

An 15N study of the effects of nitrate, ammonium, and nitrate + ammonium nutrition on nitrogen assimilation in Zea Mays L.

Bibliography: leaves 113-134.A brief review of the literature on the effects of nitrate and ammonium nitrogen sources on plant growth, and the assimilation of those nitrogen sources, has been presented. A water culture technique for the growth of maize was developed. The use of a gravel rooting medium with nutrients recycling through the rooting medium, and an iron supplement in the form of ferric citrate, resulted in superior growth of nitrate-fed plants in comparison to that observed with a hydroponic growth technique and a standard Long Ashton nutrient solution. The effects of nitrogen source on plant growth, organic nitrogen and inorganic nitrogen contents, and the rates of incorporation into nitrogenous compounds were studied. The observed differences were explained with reference to the effects of the various nitrogen sources on the physiology of the plants. The experimental techniques included assays of the enzymes nitrate reductase and glutamine synthetase, whole plant growth studies, and the analysis of nitrogenous compounds of xylem exudate and those extracted from the leaf blade, leaf base, and root regions of maize plants after feeding with a nutrient solution containing nitrogen as 15N